Inhibition of TRPM7 suppresses migration and invasion of prostate cancer cells via inactivation of ERK1/2, Src and Akt pathway signaling

Background: Prostate cancer is the second most common cause of related death in males worldwide. Most patients show no response to androgen deprivation therapy case recurrence and proceed advanced stage with metastasis. TRPM7 reported be upregulated diverse types tumors. Methods: We analyzed expression proteins by Western blotting analysis. performed cell migration invasion assay analyze relationship between tumor aggressiveness TRPM7. In addition, we proceeded an animal study using stable knockdown line xenograft. Results: our results, regulates prostate biology including proliferation, through ERK1/2, PI3K/Akt JNK signaling pathways. produced line. To tumorigenesis, as well xenograft model. down-regulated DU145 cells showed suppressed migratory ability, 0.65- 0.05-fold, respectively. confirmed that anti-cancer effect mediated inactivation Src Akt pathways western P-ERK1/2, p-Src, p-Akt expressions were reduced 0.66-, 0.68-, 0.66-fold, Moreover, treated ERK, inhibitors clarify involvement each protein could observe inhibitor ability. vivo, projected decreased proliferation rate. Conclusions: Taken these results together, out suggested might essential gene for metastasis regulating